Cells Volume (Biovolume) One of several common methods used to estimate biomass of algae in aquatic systems. Cell members of algae are frequently used in aquatic surveys as an indicator of algal production. However, cell numbers alone cannot represent true biomass because of considerable cell-size variation among the algal species. Cell volume (µm3) is determined by obtaining critical cell measurements on cell dimensions (for example, length, width, height, or radius) for 20 to 50 cells of each important species to obtain an average biovolume per cell. Cells are categorized according to the correspondence of their cellular shape to the nearest geometric solid or combination of simple solids (for example, spheres, cones, or cylinders). Representative formulae used to compute biovolume are as follows: (1) sphere: 4/3 ðr2 , (2) cone: 1/3 ðr3h, (3) cylinder: ðr3h. From cell volume, total algal biomass expressed as biovolume (µm3/mL) is thus determined by multiplying the number of cells of a given species by its average cell volume and then summing these volumes overall all species.
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